Oligo Explorer 1.5Free
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Oligo Explorer was developed for molecular biologists who want to design their PCR and sequencing primers by themselves. Oligo Explorer is an efficient easy-to-use tool to determine primer properties like Tm, GC%, primer loops and primer dimers.
Oligo Explorer also includes a powerful 'Primer wizard' tool that helps you to find suitable primer pairs for your purposes. You can set your own parameters for primer pair search engine or use default parameters.
Default parameters are usually adequate for most applications. 'Primer wizard' suggests primer pairs that amplify PCR-product of given length. Individual primer pairs have been tested so that they do not form stable primer dimers or primer loops.